Goat Simulator Free的問題,透過圖書和論文來找解法和答案更準確安心。 我們挖掘出下列價位、菜單、推薦和訂位總整理
另外網站Goat Simulator Free on Windows PC Download Free - 1.99也說明:Download and play Goat Simulator Free 1.99 on Windows PC. Play the new goat simulator game for FREE. Walk and run through the fields.Let it goat, Let it ...
國立臺灣大學 動物科學技術學研究所 陳明汝所指導 凃博文的 探討荷蘭乳牛消化道微生物組與乳房炎之關聯性 (2017),提出Goat Simulator Free關鍵因素是什麼,來自於荷蘭乳牛、腸道微生物組、乳房炎、代謝體、瘤胃。
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Goat Simulator Free進入發燒排行的影片
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探討荷蘭乳牛消化道微生物組與乳房炎之關聯性
為了解決Goat Simulator Free 的問題,作者凃博文 這樣論述:
乳房炎被認為是造成酪農業產業巨大經濟損失的疾病,儘管其後發展出各種防治乳房炎的方法,但抗生素治療和牧場衛生管理仍是牧場中防治乳房炎的主要策略。然而,病原菌抗藥性及控制乳房炎的效率低落都一再促使人們研究替代方案,希望能維持動物健康。目前已有諸多研究指出消化道微生物組對動物健康狀態之變化也非常敏感,但是泌乳牛的消化道微生物組成與乳房炎之間關聯性的相關研究仍相當稀少。因此,本研究的目的即是評估荷蘭乳牛消化道微生物組成與乳房炎之間的相關性。本研究選用16頭泌乳牛,並將其分為健康泌乳牛組別和乳房炎泌乳牛組別,接著利用次世代基因定序法以及高效能液相層析串聯式質譜儀各別分析消化道微生物組與消化道代謝體。根
據次世代基因定序結果可得知,健康與乳房炎泌乳牛兩組組內的消化道菌相之物種豐富度與多樣性相似,而瘤胃優勢細菌菌屬為Prevotella_1 (8.58%) ,其次依序為Succinivibrionaceae_UCG-001 (7.87%) 、Ruminococcaceae_NK4A214_group (6.60%) 、Christensenellaceae_R-7_group (5.75%) 、Ruminococcus_2 (2.87%) 與Ruminococcus_1 (2.86%) ;瘤胃優勢原蟲屬則是Entodinium (56.39%) 、Isotricha (9.57%) 、Diplo
dinium (2.50%) 與Dasytricha (2.50%) ;糞便優勢細菌菌屬為SMB53 (4.66%) ,其次依序是Butyrivibrio (3.02%) 、Oscillospira (3.01%) 、5-7N15 (2.59%) 與Clostridium (2.16%) 。更進一步,本研究以偏最小平方判別分析法 (partial least squares discriminant analysis, PLS-DA) 分析兩組間瘤胃與糞便消化道微生物相是否具有組間差異,所得結果顯示出兩組之間的瘤胃細菌菌相有完全的分群效果,即為兩組別間瘤胃細菌菌相組成有所差異。因此,本研究即利
用線性判別分析效力量鑑別分析 (linear discriminant analysis effect size analysis, LEfSe) 從菌門階層開始比較分析並逐步限縮至菌種層級,找出乳房炎與健康泌乳牛各自具生物標記功能之細菌菌群,首先在乳房炎泌乳牛中找到三個分類群分別是Bacillus (菌屬) 、Sharpea (菌屬) 與Bacillus anthracis (菌種) ;在健康泌乳牛中則找到七個分類群分別是Selenomonas_1 (菌屬) 、Fibrobacter (菌屬) 、Treponema_2 (菌屬) 、Ruminococcus_1 (菌屬) 、Ruminococ
caceae_UCG-014 (菌屬) 、Ruminococcus flavefaciens (菌種) 與Treponema saccharophilum (菌種) 。為了深入探討生物標記菌與乳房炎指標 (血清介白素-6與乳體細胞數) 之關聯性,本研究利用Spearman秩相關係數 (Spearman’s correlation coefficient) 分析可得知,血清介白素-6與乳中體細胞數跟乳房炎組生物標記 (Bacillus、Sharpea與Bacillus anthracis) 呈現正相關性;與健康組生物標記 (Ruminococcaceae_UCG-014、Ruminococcus f
lavefaciens與Treponema saccharophilum) 呈現負相關性。另外,透過代謝體分析可得知,共21種化合物在健康泌乳牛的含量高於乳房炎泌乳牛 (P < 0.05) ,最後本研究亦利用Spearman秩相關係數分析瘤胃細菌與瘤胃代謝物之相關性。目前本研究找出具有潛力的生物標記菌群以及泌乳牛中消化道微生物組與乳房炎之相關性,本研究或許能在防治乳房炎的策略上提供一個全新的方向。
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